Composite

Part:BBa_K3590040:Design

Designed by: Barbora Hrnčířová   Group: iGEM20_Brno_Czech_Republic   (2020-10-19)


Scaffoldin for degradation of microcystin - MC-LR


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1612
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1612
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1612
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1612
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2811
    Illegal BsaI.rc site found at 306


Design Notes

For cloning purposes, attaaGAATTC (containing EcoRI) was added to 5' end and tttAAGCTT (containing HindIII) was added to 3' end. On both ends of the sequence, there are several nucleotides that help the restriction enzymes to interact with the sequence.


Source

This is a synthetic part. The sources of each fragment can be found at the individual page of each Basic Part that is present in this Composite Part.

References